‘Ainatul Soraya Ramzani, Aishah Nurain Binti Zulazli, Hang Nur Deanna Sofea Binti Hang Al Nuqman Shah, Siti Marziah Binti Mohd Dani and Muhammad Amir bin Yunus
Keywords: Alocasia denudate stem, crude extract, fibroblast cells, in vitro, wound healing
Abstract: Among most essential factors in wound healing pathways is transforming the vascular endothelial growth factor. Fibroblasts are the main cells in all stages of wound closure. In this research, the crude extract of Alocasia denudata from its stem was used to treat the DF-1 chicken fibroblast cells as they were also adherent cells, similar to human fibroblasts. Firstly, the cytotoxicity of the Alocasia denudata stem crude extract was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay or commonly known as MTT assay. Using a suitable range of concentration of the crude extract, we then conducted the cell migration assay to assess the migration rate of the DF-1 chicken fibroblast cells in response to the crude extract. The rates of cells migration process was monitored for 48 h. We found that the crude extract of Alocasia denudata stem at 50 (μg/mL) was able to promote the migration of chicken fibroblast cells in vitro. Based on this research outcome, we propose that the raw stem juice of Alocasia denudata could have a stimulatory effect in wound healing process.