Zarina Zakaria, Nur Zatul ‘Iffah Zakaria, Low Jia Zhen and Chong Shi Fern

Keywords: Grey Oyster Mushroom, Liquid Spawn, Solid Spawn

Abstract: In mushroom industry, grey oyster mushroom (Pleurotus pulmonarius) is commonly cultivated by using solid spawn. In the present study, liquid spawn is proposed as an alternative to replace solid spawn because solid spawn takes longer time for mycelium run
in the bag and the final yield is low. Liquid spawn was prepared by culturing spawn in Potato Dextrose Broth while solid spawn was prepared by culturing spawn in grains. This study aims to investigate the growth kinetics and morphology of P. pulmonarius on sawdust substrate by liquid spawn and comparison was made with solid spawn which act as control. Screening of parameters variables was performed using OFAT to determine the effect of the parameters on the mycelial growth rate and morphology. The parameters studied were seed culture storage condition, seed culture storage period and liquid spawn inoculum volume. The storage condition was set at cold 4 C and ambient 25 C2 C, while the storage period used was 1, 2 and 3 months. As for the inoculum volume used was 3ml, 5ml and 7ml. From these parameters, they were arranged into 18 combinations. From the findings, it was discovered that the seed culture stored at 4°C for 1 month with 7ml of liquid spawn inoculum volume demonstrated the highest mycelial growth rate (0.3946 cm/day) and highest average weight of fruiting bodies (123.0 g). Also, the average diameter of caps (12.6 cm) yield from this spawn is the highest. Besides, liquid spawn took only 48 days for complete spawn run while solid spawn took 57 days for complete spawn run. Hence, liquid spawn has the potential to be commercialized for the advantages it has over solid spawn, for instance, shorter spawn run period and higher yield.

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